Figure 2.

ncl-1 is an upstream translational suppressor of fib-1 and dao-5. The amount of FIB-1 and DAO-5 present can be correlated with the size and function of the nucleolus. (A) Comparison of nucleolar size in embryos of various genetic backgrounds at the 4–cell stage. The nucleolus of ABp cell is highlighted and magnified in the insets. Scale bar, 20 μm. (B) Western blot analysis shows the amount of fibrillarin (FIB-1), which is correlated with the nucleolar size shown in (A). Actin serves as the loading control. (C) Quantitative representation of the nucleolar size from (A). Figures (A–C) are partially adapted and modified from Yi et al., (2015)²³ with permission of PLoS Genetics. (D) Total RNAs were extracted from embryos of wild-type (N2) and ncl-1 mutants and fractionated by centrifugation in a sucrose gradient for polysome profile analysis. The amounts of fib-1 and dao-5 mRNA in each fraction were then determined by RT-qPCR and indicated by solid line (N2) or dotted line (ncl-1). The ribosome fractions from low to high sucrose gradients are indicated from left to right, representing a transition from monosomes to polysomes.