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. 2016 Feb 22;7(1):84–102. doi: 10.1080/19491034.2016.1150397

Figure 1.

Figure 1.

PL-1C7 monoclonal antibody specifically recognizes the lamin A precursor, prelamin A. (A). Diagram of prelamin A structure and processing. The PL-1C7 monoclonal antibody was raised using as an antigen a synthetic peptide composed of the 12 amino acids spanning the ZMPSTE24 cleavage site in prelamin A (T643-R654), and includes 4 amino acids from the mature lamin A as well as 8 specific prelamin A residues (gray shading). (B). PL-1C7 antibody binding to the carboxyl terminus of prelamin A was confirmed by western blot using a GST_prelamin A V629-M664 fusion protein, right lane; non-fusion protein GST control, left lane. (C). PL-1C7 epitope mapping was done by ELISA immunoassays using a panel of 7 synthetic peptides where wild type amino acids triplets were sequentially replaced by alanine triplets, as well as 2 peptide mimics of ZMPSTE24-generated lamin A fragments: pLA_Mat (G635-Y646) and pLA_frag (L647-S657). Antibody binding was plotted as percentage of binding in relation to the wild type lamin A peptide (pLA_WT). p < 0.005. See also Fig. S1.