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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Pigment Cell Melanoma Res. 2007 Jul 28;21(5):534–544. doi: 10.1111/j.1755-148X.2008.00491.x

Figure 4.

Figure 4

BCL-2 overexpression protects WM793 cells from apoptosis induced by CI-1040 combined with serum deprivation. (A) WM793 cells engineered to overexpress BCL-2 were treated with 2 μM CI-1040 for 48 h. The expression of BCL-2, BIM-EL and ERK2 was analyzed by Western blot analysis of cell extracts. (B) Vector control and BCL-2 overexpressing WM793 cells were plated at 105 cells per well and then subjected to TFD in the absence or presence of 2 μM CI-1040 for 48 h. Cells were photographed under phase-contrast microscopy (magnification 100×). (C) Vector control (black bars) and BCL-2 overexpressing (white bars) WM793 cells were plated at 105 cells per well (line represents plating density). Cells were then subjected to TFD in the absence or presence of 2 μM CI-1040 for 48 h. Cells were harvested and viable cells were determined by Trypan blue exclusion. Values represent mean ± standard deviation.