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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Pigment Cell Melanoma Res. 2007 Jul 28;21(5):534–544. doi: 10.1111/j.1755-148X.2008.00491.x

Figure 6.

Figure 6

Inhibition of BIM-EL expression by RNA interference provides WM793 protection from apoptosis. (A) Parental WM793 cells (NA) or WM793 cells carrying an empty vector control (4.1), or two different WM793 clones expressing an shRNA designed to inhibit BIM expression (4.3 and 4.5) were treated with 2 μM CI-1040 for 24 h. The effects of CI-1040 and the anti-BIM shRNA on the phosphorylation of ERK1/2 or the expression of BIM-EL was assessed by Western blot analysis of cell extracts. (B) Cells described in (A) were treated with 2 μM CI-1040 in the absence of serum for 24 or 48 h as indicated. The presence of apoptotic cells was assessed by co-staining with Annexin V and propidium iodide followed by flow cytometry as described in Materials and Methods. Results shown are representative of three independent experiments.