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. 2016 Jun 22;3:16043. doi: 10.1038/mtm.2016.43

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Diagram of the original and modified microcell-mediated chromosome transfer (MMCT) techniques. (a) The original protocol using Colcemid and Cytochalasin B as key chemicals. The cells are cultured on standard treated tissue culture plastic. (b) The protocol is identical to the original aside from Colcemid being replaced by TN-16 + Griseofulvin combination. (c) The protocol is the same as the original protocol aside from Latrunculin B replacing Cytochalasin B. (d) A modified MMCT protocol that includes replacement of both key chemicals by TN-16 + Griseofulvin and Latrunculin B. The cells are culture on plastic coated with Collagen/Laminin. Red numbers at the bottom of each panel indicates the relative increase in efficiency compared with the original procedure. Steps 1, 2, and 3 correspond to steps in the modified protocol of MMCT described in detail in Supplementary Materials.