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Immunostaining of primary PTECs at passage 3. PTECs stain positive for epithelial cell markers CK8/18, EMA, CK AE1/3, and C5α receptor, as well as for the proximal tubular marker L-FABP. Meanwhile, PTECs stain negative for fibroblast marker α-SMA (DAB staining, magnification 400 ×).
