Supplementary Figure 3.

Relative expression levels of TP53 and CDKN1A were determined by RT-qPCR. Levels of three different PTECs are grouped as untreated and NT-PTECs on day 6 (WT-day 6), untreated and NT-PTECs on day 16 (WT-day 16), and SETD2 KD-PTECs on day 25 (KD-day 25). Results are presented as 2^−∆Ct of three independent experiment using RP II as housekeeping gene. Values shown are mean ± SD. Significant differences between WT-day 6 and KD-day 25 as compared with WT-day 16 are determined by one-way ANOVA with Dunnett multiple testing correction that both WT-Day6 and KD-day25 are compared with WT-day 16. *P < .05.