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Expression levels of CDKN2A and E2F1 in HEK293T cells after SETD2 depletion. HEK293T cells were transduced with NT shRNA or with SETD2 targeting shRNAs (sh1 and sh2). Total RNA was isolated from sorted cells, and the mRNA level of CDKN2A and E2F1 was determined by RT-qPCR. Results are presented as 2−∆Ct values of two independent experiment using HPRT as endogenous control (mean ± SD).
