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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Eur J Immunol. 2012 Aug 8;42(10):2667–2682. doi: 10.1002/eji.201142161

Figure 9. HES treatment at challenge suppresses type 2 response markers.

Figure 9

Cell-free BAL supernatants from the experiment shown in Figure 8 B-D were tested (A, B) by CBA for levels of (A) IL-4 and (B) IL-5, and (C, D) by ELISA for levels of (C) RELM-α and (D) Ym1 protein. (E-H) RNA was prepared from lung tissue and qRT-PCR used to assess levels of (E) Arginase-1, (F) AMCase, (G) RELM-β and (H) eotaxin transcription relative to GAPDH. Results are representative of 2 repeat experiments, each with 4-6 mice per group. Error bars are SEM. * p<0.05, **p<0.01, ***p<0.001, one-way ANOVA, with a Tukey’s post test.