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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Neurochem Res. 2013 Oct 2;38(12):2490–2502. doi: 10.1007/s11064-013-1162-5

Figure 2. Neurofascin 155 paranodal clusters are extracted from CST KO but not from CST WT spinal cords.

Figure 2

Spinal cords from WT mice incubated in extraction buffer without detergent (A), with Triton X-100 (B) or with CHAPS (C) revealed no difference in the number of Nfasc155 paranodal clusters (D) indicating that these paranodal clusters are strongly associated in the myelin membrane. In contrast, spinal cords from CST KO mice incubated in extraction buffer containing either Triton X-100 (F) or CHAPS (G) revealed a significant reduction in cluster number (H) compared to spinal cords incubated in extraction buffer lacking detergent (E). These findings indicate that sulfatide is not essential for cluster formation; however, in the absence of sulfatide, Nfasc155 domains are highly vulnerable to degradation. Magnification bar = 15µm