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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Biomaterials. 2015 Sep 12;73:70–84. doi: 10.1016/j.biomaterials.2015.09.017

Figure 2. 3DTEBM cultures promote MM cell proliferation better than 2D and commercially available 3D systems.

Figure 2

A) Growth of MM cell lines i) MM1S, ii) H929, and iii) RPMI8226 in multi-culture with MM-derived stromal cells and endothelial cells in classic 2D cultures or in the 3DTEBM at days 3 and 7; (*) p< 0.02. B) Confocal microscopy images of multi-cultures of MM1s-GFP (green), MM-derived stroma-DiD (red) and endothelial cells-DiI (cyan) in the 3DTEBM at 1, 3, 5, and 7 days of culture, shown from 3 perspectives: Z-Stack rotated view, top down view, and bottom up view; Scale bar= 100 μm. C) Growth of MM1s in multi-culture with MM-derived stromal cells and endothelial cells in classic 2D cultures, PLGA microspheres, AlgiMatrix, Matrigel, and inside 3DTEBM at days 3 and 7 compared to day 0, (*) p< 0.01, (**) p< 0.05. D) Growth of i) primary fresh CD138+ plasma cells from three MM patients, ii) primary frozen CD138+ cells from three additional MM patients, in multi-culture conditions in classic 2D cultures (blue) or in the 3DTEBM (red), iii) and primary fresh in PLGA microspheres (green), AlgiMatrix (light green), and Matrigel (dark green) at days 3 and 7; bold line reflects average growth in the different conditions.