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. 2016 May 13;9(3):203–210. doi: 10.1016/j.tranon.2016.03.007

Figure 3.

Figure 3

RNA interference targeted to GGT1. (A) Confocal fluorescence imaging of lung cancer cell lines. Cells were transfected with GGT1 siRNA (siRNA 1 and siRNA 2) or control siRNA, followed by incubation with 1 μM gGlu-HMRG for 30 minutes. Scale bar represents 100 μm. (B) Relative GGT1 mRNA expression level of lung cancer cell lines transfected with GGT1 siRNA (siRNA1 and siRNA2) or control siRNA measured by qRT-PCR. hHPRT1 was used as a reference gene. GGT1 mRNA contents of A549, H460, and H441 transfected with siRNA 1 and siRNA2 were significantly suppressed compared with those transfected with control siRNA respectively (P < .001-.05). In contrast, there was no significant difference in GGT1 mRNA contents of H82 and H226 (P < .05, P = .20-.99). Bar represents relative GGT1 mRNA contents (A.U.) + standard deviation.