Table 6. Effects of cryoprotective agents on cell survival of BF052 during freeze-drying (FD) and storage.
| Cryoprotectants | Cell viability (log CFU/mL ± SD) | Cell viability after storage in refrigerator (log CFU/mL ± SD) | Cell viability after storage at room temperature (log CFU/mL ± SD) | |||||
|---|---|---|---|---|---|---|---|---|
| Before FD | After FD | 1 month | 3 months | 6 months | 1 month | 3 months | 6 months | |
| DI water | 9.32 ± 0.07a | 8.60 ± 0.2-b | 8.42 ± 0.02b | 8.10 ± 0.10c | 7.79 ± 0.04d | 6.09 ± 0.08e | - | - |
| 10% Sucrose | 9.32 ± 0.27a | 9.02 ± 0.01b | 8.73 ± 0.01c | 8.68 ± 0.01cd | 8.44 ± 0.02d | 6.26 ± 0.19e | -π | - |
| 10% Lactose | 9.34 ± 0.22a | 8.99 ± 0.10b | 8.75 ± 0.02bc | 8.76 ± 0.08bc | 8.65 ± 0.04d | 7.80 ± 0.03e | 6.02 ± 0.16f | 3.10 ± 0.04g |
| 10% Skim milk | 9.21 ± 0.07a | 9.16 ± 0.02a | 9.16 ± 0.01a | 9.15 ± 0.01a | 9.12 ± 0.01a | 8.78 ± 0.10b | 7.42 ± 0.08c | 6.06 ± 0.03d |
| 10% Germinated brown rice | 9.81 ± 0.32a | 9.41 ± 0.23ab | 9.19 ± 0.12bc | 8.90 ± 0.10c | nd | 7.61 ± 0.19d | 5.14 ± 0.13e | nd |
| 10% Black sesame | 9.36 ± 0.03a | 8.48 ± 0.65b | 8.39 ± 0.06b | 8.31 ± 0.08b | nd | 7.76 ± 0.16b | 6.58 ± 0.15c | nd |
| soy milk | 9.20 ± 0.27a | 8.93 ± 0.10ab | 8.87 ± 0.09ab | 8.83 ± 0.09ab | nd | 8.77 ± 0.07ab | 7.33 ± 0.14c | nd |
- The equal superscript lowercase letters indicate no significant differences between cryoprotectant (p>0.05)
π No growth
nd Not determined.