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. 2016 Jun 22;11(6):e0157772. doi: 10.1371/journal.pone.0157772

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© 2016 Ma et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A. The panels in this section show the gating strategy employed for the analysis of γδ T cells and subsets. Peripheral venous blood derived leukocytes were stained with different fluorescent antibodies and after lysis of red blood cells, the remaining cells were gated on living lymphocytes, then gated on CD3⁺γδTCR⁺ or CD3⁺γδTCR⁺γ9TCR⁺ cells, and then further gated on CD4^-CD8^- cells, respectively. B—I. Flow cytometry results are represented as the scatter dot plots of γδ T cells and subsets in healthy controls (HC) or in SLE patients before and after therapy (treatment time is indicated). SLE patients are further grouped as responders (R) and non-responders (NR) based on response to therapy.