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. Author manuscript; available in PMC: 2016 Dec 17.
Published in final edited form as: Neuroscience. 2015 Oct 26;311:253–267. doi: 10.1016/j.neuroscience.2015.10.041

Figure 2.

Figure 2

Validation of the RNAscope method using a positive and a negative control probes. A–B. In situ hybridization was performed with an RNAscope probe targeting the eukaryotic housekeeping gene Ppib (i.e., positive control). The hippocampal section was made from a CB2R WT mouse and the images were taken from the CA1 region (A) and dentate gyrus (B). C– D. The RNAscope hybridization was conducted with a negative control probe targeting the bacterial gene DapB. The CA1 region (C) and dentate gyrus (D) were imaged from a hippocampal slice cut from a WT mouse. pyr, stratum pyramidale; rad, stratum radiatum; mol, stratum moleculare; gra, stratum granulosum; hil, hilus.