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. 2016 Jun 22;11(6):e0157214. doi: 10.1371/journal.pone.0157214

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© 2016 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 11 — A: The 3D bioprinted construct before implantation. B: The degraded material without hASCs. C: The 3D bioprinted construct containing hASCs. D: H&E staining of paraffin-embedded sections at 8 weeks after implantation showing obvious new bone matrix formation. E: Masson trichrome staining showing new bone matrix formation. The arrow indicatesblood vessels. F: OCN immunohistochemical staining showing obvious OCN expression in the surrounding cells (see S4 and S5 Figs).