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. 2016 Jun 22;11(6):e0158009. doi: 10.1371/journal.pone.0158009

Fig 2. Expression of full-length antibodies from recombinant AAV vectors.

Fig 2

Levels of expressed IgG or immunoadhesin were analyzed by Western Blot after transfection of HEK293T cells with equal amounts of plasmid DNA (0.5 μg + 0.5 μg or 1 μg). Comparison of secreted (A) 4L6 IgGs or (B) 5L7 IgGs from co-transfection of heavy and light chain vectors (two vector approach) vs. transfection of bicistronic vectors (one vector approach). The two vector approach yielded slightly higher levels of secreted antibodies than the one vector approach. IgG1 versions of the 4L6 and 5L7 full-length antibodies expressed better than IgG2 versions.