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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: J Proteome Res. 2011 Mar 24;10(5):2417–2424. doi: 10.1021/pr1012398

Figure 1.

Figure 1

SDS-PAGE and PMF analysis of purified OM from B. pseudomallei. Ten migrograms (lane 1) and 40 μg (lane 2) of protein from a Triton-insoluble OM pellet that had been further purified by extraction with RNase, Na2CO3, and NaCl were boiled for 5 min in denaturing solution (10 mM Tris-HCl pH 6.8, 2% SDS, 2% mercaptoethanol) and electrophoresed at 100 V for 3 h on a 4 to 20% polyacrylamide gradient gel (Thermo Fisher 25204). After Coomassie Blue staining, bands were excised and the protein species present in them were identified by PMF. Locus tags of PMF-identified OMPs are on the left; migration of molecular weight markers is shown on right. Cells were grown in medium 2.