Table 1.
Twenty Most Abundant OM Proteins of B. pseudomallei Detected Under All Growth Conditionsa
Bp locus tag | locus tag of Bm ortholog | predicted function | COG | % of total OMPsb | detected in Bm OM | % of total OMPsc |
---|---|---|---|---|---|---|
BPSL2559 | BMA2089 | porin | 3203 | 18.8 | yes | 6.82 |
BPSL2989 | BMA2507 | lipoprotein | 3133 | 13.0 | yes | 5.23 |
BPSL2704 | BMA2010 | OmpW-family protein | 3407 | 5.88 | yes | 0.37 |
BPSS0879 d | BMAA1353 | porin | 3203 | 4.63 | yes | 11.0 |
BPSS1356 | BMAA0912 | hypothetical protein | 3246 | 3.69 | yes | 1.10 |
BPSL2522 d | BMA0436 | OmpA-family protein | 2885 | 3.53 | yes | 7.63 |
BPSL2003 | BMA0904 | hypothetical protein | 0393 | 1.78 | yes | 1.05 |
BPSS1679d | BMAA1698 | porin | 3203 | 1.67 | yes | 1.47 |
BPSS0943 | BMAA1286 | porin | 3203 | 1.42 | yes | 3.10 |
BPSL1029 | BMA0743 | porin | 3203 | 1.08 | yes | 1.47 |
BPSL1775 d | BMA1178 | uptake receptor for Fe-catechol complex | 4774 | 0.79 | yes | 3.89 |
BPSL3036 | BMA2562 | porin | 3203 | 0.77 | yes | 1.06 |
BPSL3161 | BMA2738 | OmpW-family protein | 3047 | 0.61 | no | 0.50 |
BPSL0816 | BMA0317 | OprB OM efflux protein | 1538 | 0.58 | yes | 0.48 |
BPSL3105 | None | T6SS-associated HCP protein | 3157 | 0.30 | n/a | 0.48 |
BPSL3107 | None | T6SS-associated protein | 3516 | 0.29 | n/a | 0.22 |
BPSL0289 | BMA3345 | porin | 3203 | 0.26 | yes | 1.87 |
BPSL0007d | BMA2786 | general secretory pathway secretin GspD | 1450 | 0.20 | yes | 0.22 |
BPSL0294 | BMA3354 | selective porin | 3659 | 0.19 | yes | 1.25 |
BPSL1913d | BMA1056 | OM lipoprotein in NodT family | 1538 | 0.20 | no | 0.63 |
Bold indicates proteins also detected by SDS-PAGE and PMF (Fig. 1). Locus tags, COG assignments, and predicted function were derived from Integrated Microbial Genomes (IMG: http://img.jgi.doe.gov/cgi-bin/pub/main.cgi).
Percent of total OMPs was determined by spectral counts from cells grown in M9 minimal media with 3% glycerol.
Percent of total OMPs was determined by spectral counts from cells grown in M9 media with 3% glycerol and 20 amino acids.