Table 1.

Twenty Most Abundant OM Proteins of B. pseudomallei Detected Under All Growth Conditions^a

Bp locus tag	locus tag of Bm ortholog	predicted function	COG	% of total OMPsb	detected in Bm OM	% of total OMPsc
BPSL2559	BMA2089	porin	3203	18.8	yes	6.82
BPSL2989	BMA2507	lipoprotein	3133	13.0	yes	5.23
BPSL2704	BMA2010	OmpW-family protein	3407	5.88	yes	0.37
BPSS0879 d	BMAA1353	porin	3203	4.63	yes	11.0
BPSS1356	BMAA0912	hypothetical protein	3246	3.69	yes	1.10
BPSL2522 d	BMA0436	OmpA-family protein	2885	3.53	yes	7.63
BPSL2003	BMA0904	hypothetical protein	0393	1.78	yes	1.05
BPSS1679d	BMAA1698	porin	3203	1.67	yes	1.47
BPSS0943	BMAA1286	porin	3203	1.42	yes	3.10
BPSL1029	BMA0743	porin	3203	1.08	yes	1.47
BPSL1775 d	BMA1178	uptake receptor for Fe-catechol complex	4774	0.79	yes	3.89
BPSL3036	BMA2562	porin	3203	0.77	yes	1.06
BPSL3161	BMA2738	OmpW-family protein	3047	0.61	no	0.50
BPSL0816	BMA0317	OprB OM efflux protein	1538	0.58	yes	0.48
BPSL3105	None	T6SS-associated HCP protein	3157	0.30	n/a	0.48
BPSL3107	None	T6SS-associated protein	3516	0.29	n/a	0.22
BPSL0289	BMA3345	porin	3203	0.26	yes	1.87
BPSL0007d	BMA2786	general secretory pathway secretin GspD	1450	0.20	yes	0.22
BPSL0294	BMA3354	selective porin	3659	0.19	yes	1.25
BPSL1913d	BMA1056	OM lipoprotein in NodT family	1538	0.20	no	0.63

^aBold indicates proteins also detected by SDS-PAGE and PMF (Fig. 1). Locus tags, COG assignments, and predicted function were derived from Integrated Microbial Genomes (IMG: http://img.jgi.doe.gov/cgi-bin/pub/main.cgi).

^bPercent of total OMPs was determined by spectral counts from cells grown in M9 minimal media with 3% glycerol.

^cPercent of total OMPs was determined by spectral counts from cells grown in M9 media with 3% glycerol and 20 amino acids.

^dReported to reacted with sera from Bp infected patients.^22,23 n/a, not applicable.