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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Nat Protoc. 2015 Oct 22;10(11):1860–1896. doi: 10.1038/nprot.2015.122

Figure 10.

Figure 10

Two different workflows for cell tracing in neuTube and Imaris. (a) Tracing using neuTube67. (b) Tracing using Imaris 7.1 (Bitplane). Results shown here took 25 min for a novice user with ~5 h of total experience using each tracing tool. Total tracing time to achieve similar results was generally comparable, but we found neuTube to be more efficient for quickly tracing isolated neurites. (a) neuTube 3D visualization (i), neuTube semiautomated tracing result (ii), tracing error (iii), and manual correction (iv). (b) Imaris ROI selection (i), Imaris `Autopath' seeding (ii), manual correction of tracing error (iii), and trace extension using `Autopath' (iv). The original test mage on which semiautomated tracing was performed is provided in Supplementary Data 3.