HSC were isolated from Smoflox/flox mice, and treated for 3 days with adenovirus bearing Cre recombinase (Ad-Cre) or adenovirus expressing green fluorescent protein (Ad-GFP, control). Cells were harvested for mRNA and protein analysis by qRT-PCR or western blot respectively. (A) Smo and Gli1 mRNA. (B) Yap1 mRNA. (C) Representative western blot showing pYap1 S112 Yap1, pLats1 T1079, Lats1, and beta-actin. (D) CTGF mRNA. (E) AREG mRNA. Results were expressed as fold change relative to control (Ad-GFP) treated HSC; mean ± SEM were graphed. *p<0.05 vs. control