. Author manuscript; available in PMC: 2017 Jul 1.

Published in final edited form as: DNA Repair (Amst). 2016 May 13;43:48–56. doi: 10.1016/j.dnarep.2016.05.029

Table 2.

Effects of point mutations on AIDv(Δ15) activity and processivity

Enzyme	Activity^*	Number of C→U	p-value^**
AIDv(Δ15)	1	10
N51A	0.04	3.7	< 0.01
K52A	0.75	5.8	< 0.01
Y114A	0.05	2.3	< 0.01
F115A	0.44	5.7	< 0.01
C116A	0.35	9.5	0.68
E117A	0.13	8	0.14
R119A	0.69	15.2	< 0.01
K120A	1.1	11.7	0.23
E122A	0.24	7.1	0.06
R171A	0.17	5.3	< 0.01
Q175A	0.47	5.3	< 0.01
R178A	0.73	4.7	< 0.01

Deamination activity was measured using the lacZa gap deamination assay.

Relative activities were calculated as the ratio of the frequency of M13 mutant plaques for each AIDv(Δ15) mutant to the frequency of M13 mutant plaques for AIDv(Δ15).

^**

P-values for comparison of mutants to AIDv(Δ15) processivity were calculated using an unpaired t-test.