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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Cell Rep. 2016 Jun 9;15(12):2719–2732. doi: 10.1016/j.celrep.2016.05.058

Figure 4. Spontaneous tumors in KPC mice respond to Gem/nP/αCD40 therapy in T cell-dependent fashion.

Figure 4

KPC mice diagnosed with established tumors received Gem/nP on day 0 and day 7, and αCD40 was given on day 2. Some mice (as indicated) also received αCD8 depletion for the duration of enrollment. (A) The change in tumor volume on day 14 compared to initial tumor volume at diagnosis, responders calculated in table below.

(B) Tumor growth curves for indicated groups, responders indicated in red.

(C) Representative histological samples from (A) at day 14, stained for CD8, shown on left at two magnifications, quantification of global CD8 staining in tumors on right. Scale bar indicates 200 µm (top) or 300 µm (bottom).

(D) Representative H&E samples of tumors from (A) at day 14 shown on left, quantification of tertiary lymphoid structures (TLS) in entire tumor section on right. Top, 2×, Arrowheads point to TLS, asterisk indicated tumor-associated lymph node, and outline indicates field below (20×). Scale bar indicates 1000 µm (top) or 100 µm (bottom).

Each bar, line, or symbol represents an individual mouse, horizontal lines indicate mean ± SD. Statistical analysis by one-way ANOVA with Tukey’s HSD post-test (C, D), and Fisher’s Exact Test (A).