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. Author manuscript; available in PMC: 2017 Jun 21.
Published in final edited form as: Immunity. 2016 May 31;44(6):1350–1364. doi: 10.1016/j.immuni.2016.04.013

Figure 6. SOCE controls the transcriptional programming of Tfh cells.

Figure 6

(A) Heatmap of selected transcription factors of Tfh cells and non-Tfh cells from LCMV-infected WT and Stim1fl/flStim2fl/flCd4cre (DKO) mice. (B) Expression of Nfat2 and Nfat2αA genes in Tfh cells analyzed by qRT-PCR; means ± SEM of n=4 mice. (C, D) Expression of IRF4 in Tfh and non-Tfh cells from LCMV-infected WT and DKO mice analyzed by qRT-PCR (C; means ± SEM of n=4 mice) and by flow cytometry (D; means ± SEM of n=5 mice). (E) Retroviral expression of caNFAT2 in WT and DKO CD4+ T cells in vitro and analysis of IRF4 expression by flow cytometry 5 d later. Means ± SEM of n=4 mice. (F-I) Expression of BATF (F, G) and Bcl-6 (H, I) mRNA and protein in Tfh and non-Tfh cells analyzed by qRT-PCR and flow cytometry, respectively. Means ± SEM of 4 mice (F), 6 mice (G), 4 mice (H) and 7 mice (I). (J) Analysis of BCL6 mRNA expression by qRT-PCR in T cells from healthy donors (HD) and a patient with ORAI1 p.R91W mutation. (K) Ectopic expression of caNFAT2 partially rescues Tfh cell differentiation in vivo. CD4+ T cells from SMARTA WT and DKO mice were retrovirally transduced with caNFAT2 or Bcl-6 and transferred to congenic mice infected with LCMV. Analysis of Tfh cell differentiation in donor T cells by flow cytometry; means ± SEM of 2-3 mice. See also Figure S6.