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Modest effects of Cdkn2a deficiency on PLT activation with chow feeding. A: PLT surface P-selectin levels in acid-citrate-dextrose whole blood stimulated with PAR4 agonist AYPGKF (100 uM) and analyzed by flow cytometry. B: Circulating soluble P-selectin (sP-selectin) in PLT-poor plasma by commercially-available ELISA. C, Time to cessation of tail vein bleeding following tail clip injury. One-way ANOVA (A-C). MFI, mean fluorescent intensity; NS, not significant.
