Table 2.
Inter-day recovery, precision, and accuracy.
| Desired (μg/mL) | Day 1 (calculate) | % Recovery | Day 2 (calculate) | % Recovery | Day 3 (calculate) | % Recovery | Mean (% Recovery) | SD (% Recovery) | %CV (% Recovery) | Accuracy (%RE) |
|---|---|---|---|---|---|---|---|---|---|---|
| 0.05 | 0.05 | 100.00 | 0.06 | 120.00 | 0.04 | 84.00 | 101.33 | 18.04 | 17.80 | |
| 0.05 | 0.04 | 80.00 | 0.05 | 100.00 | 0.06 | 116.00 | 98.67 | 18.04 | 18.28 | |
| 0.05 | 0.06 | 120.00 | 0.05 | 100.00 | 0.06 | 120.00 | 113.33 | 11.55 | 10.19 | |
| 0.05 | 0.05 | 100.00 | 0.05 | 100.00 | 0.06 | 120.00 | 106.67 | 11.55 | 10.83 | |
| 0.05 | 0.05 | 100.00 | 0.06 | 120.00 | 0.06 | 120.00 | 113.33 | 11.55 | 10.19 | |
| 0.05 | 0.05 | 100.00 | 0.04 | 80.00 | 0.04 | 80.00 | 86.67 | 11.55 | 13.32 | |
| Mean | 0.05 | 100.00 | 0.05 | 103.33 | 0.05 | 106.67 | 103.33 | |||
| SD | 0.01 | 12.65 | 0.01 | 15.06 | 0.01 | 19.21 | 10.15 | |||
| CV% | 12.65 | 12.65 | 14.57 | 14.57 | 18.01 | 18.01 | 9.82 | 0.00 | ||
| 0.25 | 0.20 | 80.00 | 0.23 | 92.00 | 0.28 | 112.00 | 94.67 | 16.17 | 17.08 | |
| 0.25 | 0.26 | 104.00 | 0.23 | 92.00 | 0.24 | 96.00 | 97.33 | 6.11 | 6.28 | |
| 0.25 | 0.20 | 80.00 | 0.22 | 88.00 | 0.24 | 96.00 | 88.00 | 8.00 | 9.09 | |
| 0.25 | 0.22 | 88.00 | 0.21 | 84.00 | 0.25 | 100.00 | 90.67 | 8.33 | 9.18 | |
| 0.25 | 0.20 | 80.00 | 0.23 | 92.00 | 0.23 | 92.00 | 88.00 | 6.93 | 7.87 | |
| 0.25 | 0.26 | 104.00 | 0.24 | 96.00 | 0.24 | 96.00 | 98.67 | 4.62 | 4.68 | |
| Mean | 0.22 | 89.33 | 0.23 | 90.67 | 0.25 | 98.67 | 92.89 | |||
| SD | 0.03 | 11.78 | 0.01 | 4.13 | 0.02 | 7.00 | 4.67 | |||
| CV% | 13.18 | 13.18 | 4.56 | 4.56 | 7.10 | 7.10 | 5.03 | −8.00 | ||
| 5 | 4.14 | 82.80 | 4.72 | 94.40 | 4.18 | 83.6 | 86.93 | 6.48 | 7.45 | |
| 5 | 4.12 | 82.40 | 4.21 | 84.20 | 3.98 | 79.6 | 82.07 | 2.32 | 2.82 | |
| 5 | 5.13 | 102.60 | 5.08 | 101.60 | 4.01 | 80.20 | 94.80 | 12.65 | 13.35 | |
| 5 | 4.22 | 84.40 | 4.87 | 97.40 | 4.87 | 97.40 | 93.07 | 7.51 | 8.06 | |
| 5 | 5.08 | 101.60 | 3.95 | 79.00 | 3.98 | 79.60 | 86.73 | 12.88 | 14.85 | |
| 5 | 3.82 | 76.40 | 4.14 | 82.80 | 4.21 | 84.20 | 81.13 | 4.16 | 5.13 | |
| Mean | 4.42 | 88.37 | 4.50 | 89.90 | 4.21 | 84.10 | 87.46 | |||
| SD | 0.55 | 10.98 | 0.46 | 9.11 | 0.34 | 6.82 | 5.57 | |||
| CV% | 12.43 | 12.43 | 10.14 | 10.14 | 8.11 | 8.11 | 6.37 | −12.40 | ||
| 10 | 8.26 | 82.60 | 8.82 | 88.20 | 8.09 | 80.90 | 83.90 | 3.82 | 4.55 | |
| 10 | 8.54 | 85.40 | 8.78 | 87.80 | 8.99 | 89.90 | 87.70 | 2.25 | 2.57 | |
| 10 | 7.41 | 74.10 | 10.93 | 109.30 | 8.77 | 87.70 | 90.37 | 17.75 | 19.64 | |
| 10 | 8.47 | 84.70 | 10.37 | 103.70 | 8.93 | 89.30 | 92.57 | 9.91 | 10.71 | |
| 10 | 10.93 | 109.30 | 9.23 | 92.30 | 9.21 | 92.10 | 97.90 | 9.87 | 10.08 | |
| 10 | 8.91 | 89.10 | 8.97 | 89.70 | 8.94 | 89.40 | 89.40 | 0.30 | 0.34 | |
| Mean | 8.75 | 87.53 | 9.52 | 95.17 | 8.82 | 88.22 | 90.31 | |||
| SD | 1.18 | 11.78 | 0.91 | 9.09 | 0.39 | 3.85 | 4.72 | |||
| CV% | 13.46 | 13.46 | 9.56 | 9.56 | 4.37 | 4.37 | 5.23 | −9.70 | ||
% CV = (SD/calculated concentration) × 100; % RE = (calculated concentration − desired concentration)/(desired concentration × 100).