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. 2016 Jun 23;10:167. doi: 10.3389/fncel.2016.00167

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Copyright © 2016 Sheen, Chen, Hsu, Tain, Yu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Staining of ADMA in intact cells and visualization by fluorescence microscopy. (A) Representative images acquired from cultures of rat astrocyte C6 cell lines. ADMA staining is shown in the upper panel. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI; middle panels). Merged images are shown in the lower panels. (B) The concentration of ADMA in whole cell lysates. One-way ANOVA followed by Tukey HSD post hoc test was used to assess the statistically significances among groups, *P < 0.05 vs. control; ^#P < 0.05 vs. TNF-α incubated only.