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. 2016 Jun 23;7:885. doi: 10.3389/fpls.2016.00885

FIGURE 3.

FIGURE 3

Flowers and transition from floral to vegetative phase are not required for SAP54-mediated enhancement of insect colonization. (A) M. quadrilineatus produces more nymphs on 35S:GFP-SAP54 transgenic A. thaliana (Col-0) plants with leaf-like flowers than on 35S:GFP (Col-0) control plants with wild type flowers. (B) Removal of flowers and floral stems does not affect M. quadrilineatus colonization preference of 35S:GFP-SAP54 transgenic A. thaliana (Col-0). (C) Leafhoppers also prefer GFP-SAP54 transgenic plants prior to transition from vegetative to floral growth. (D) M. quadrilineatus lays more eggs on single rosette leaves of GFP-SAP54 transgenic plants. Bars shown are one standard error of the mean. Each experiment was replicated in six independent choice cages. Significant difference in leafhopper reproductive preference are indicated as follows: ∗∗∗p ≤ 0.001; ∗∗p ≤ 0.025 (paired t-test). All experiments were repeated three times with similar results (Supplementary Figure S1A).