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. 2016 Jun 23;7:915. doi: 10.3389/fpls.2016.00915

FIGURE 1.

FIGURE 1

Selection of candidates for Pib allele mining. Accessions from 12 major rice growing countries that were chosen based on their phenotype (score 0) in UBN and against at least two of the five pure blast isolates are presented (A). The accessions that are molecular positives for Pib based on Nsb marker screening were chosen as candidates, and the candidates from which Pib was successfully amplified are also presented. Sample picture of molecular screening of rice accessions for selection of Pib allele mining candidates is shown (B). 1 kb, DNA marker; LTH, negative control; Pib, positive control (Pib-monogenic line); WC, water control; 1–11, test accessions. The accessions positive for Pib are 2 (IRGC-4471), 3 (IRGC-4552), 4 (IRGC-4553), 7 (IRGC-4574), 8 (IRGC-4619), 9 (IRGC-4633), 10 (IRGC-4634), and 11 (IRGC-4642). The upper band (629 bp) indicates the presence of Pib and the lower band (∼250 bp) shows the actin band, which is an internal control for the PCR.