Figure 3.

Anti-VEGF-A treatment does not affect CTB uptake into the retina. (a) Rat eyes were bilaterally injected IVT with 20 pmol IgG₁ or sVEGFR-2, then 7 days later with 2 μl of 0.75% CTB. The tissue was dissected, flat mounted and confocal tile scans made of entire retinas harvested at 4 (left panels), 8 (middle left panels), 24 (middle right panels) and 48 h (right panels) post-CTB injection. Original magnification= × 4. (b) Quantification of mean gray levels indicated an increase in fluorescence intensity of CTB in the retina from 4 to 24 h post-injection; however comparison of IgG₁ and sVEGFR-2 groups revealed no significant differences between treatments at any of the time points. (c) Higher magnification images of RGCs showed no variation in intracellular accumulation of CTB using × 10 (upper panels) or × 40 (lower panels) magnification at 48 h post-CTB injection, with IgG₁ (left panels) or sVEGFR-2 (right panels) treatment. White boxes on upper panels indicate regions that were imaged using × 40 objective for the lower panels. N=6–10. Data are given as means±S.E.M.