Fig. 3. Targeted recruitment of the Sin3/HDAC to long nucleosomal templates pre-acetylated with the yeast NuA4 complex.

Immobilized nucleosomal templates acetylated with the yeast NuA4 complex were incubated with or wihout LexA-Mad and subsequently incubated with the Sin3/HDAC complex in the presence or absence of competitor nucleosomal arrays. The amount of histone H4 acetylation was determined with western blotting with an antibody that recognizes tetra-acetylated histone H4.