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. 2016 May 26;7(5):e2237. doi: 10.1038/cddis.2016.111

Figure 1.

Figure 1

Decrease of FXN in granule cells and glia from YG8R mice. (A) The panel on the left shows immunofluorescence on co-cultures of granule cells and glia in the two genotypes: Y47R (control), YG8R (190 and 90 GAA repeats; FRDA model). In a and c are shown the merge of DAPI (blue), α-MAP-2 (green) and α-FXN (red), while b and d show α-hFXN (red). (B, C) The histograms represent the mean intensity of the α-hFXN antibody measured cell-by-cell. On top the histogram shows the mean of hFXN level in granule cells and on the bottom in glial cells. The fluorescence was averaged between cells and number of animals used (three independent experiments were conducted per case). In both cell types granule cells and glia, the level of FXN is significantly decreased in YG8R cells (**P<0.01; one–way ANOVA test with Bonferroni correction), compared with Y47R. (D) The figure shows the western blot on α-hFXN and α-AIF conducted on cerebella extracts of 8.5-months-old mice. (E) The histogram shows the quantification of the western blot (three independent experiments and n=3 mice; ***P=0.0004; t-test)