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. 2016 May 19;7(5):e2228. doi: 10.1038/cddis.2016.124

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Linc-UFC1 knockdown inhibited proliferation of CRC cells via cell cycle arrest. (a) Knockdown of linc-UFC1 impaired proliferation in LOVO and SW480 cells, as revealed by CellTiter 96 AQ_ueous One Solution Cell Proliferation assay (n=6; *P<0.05 versus sh#1 and sh#2). (b and c) Histological analysis of the rates of colony formation in control (NC) and linc-UFC1 knockdown groups (sh#1 and sh#2; n=6; *P<0.05 versus NC). (d) The EdU incorporation assay to examine the effects of linc-UFC1 inhibition on DNA synthesis during cell growth. The images were taken at × 200. The result showed that the proportion of S phase cells (EdU-positive cells) was decreased in shRNA-treated groups (n=6, P<0.05 versus NC). (e) Flow cytometric analysis of cell cycle arrest 48 h after treatment with shRNAs (sh#1 and sh#2) and negative control (NC) in LOVO and SW480 cells (n=6, P<0.05 versus NC). (f and g) The expression levels of cell cycle-related proteins (cyclin D1, CDK4, Rb and p-Rb) indicated by western blotting in control (NC) and linc-UFC1 knockdown groups of LOVO and SW480 cells (n=6; *P<0.05 versus NC)