Figure 5.

β-arr1 deficiency increased ISC apoptosis after 5-FU treatment. (a) Intestinal sections with the indicated genotypes were subjected to TUNEL (red) and EGFP (green, to detect Lgr5+ cells) staining. White arrows indicate double-positive signals. (b) Apoptotic Lgr5+ stem cells were counted in every 10 crypts after 5-FU treatment for 5 days. Values are shown as the mean±S.D., n=4 in each group. (c) Sections were stained for MMP7 (brown) to label Paneth cells. (d) Paneth cells were reduced after 5-FU treatment. However, β-arr1 deficiency did not affect the Paneth cells. Values are shown as the mean±S.D., n=4 in each group. (e) Sections were stained with PAS (magenta) to label goblet cells. (f) Goblet cells were not significantly reduced after 5-FU treatment, and β-arr1 deficiency did not affect the goblet cells. Values are shown as the mean±S.D., n=4 in each group