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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: AIDS. 2015 Nov;29(17):2235–2244. doi: 10.1097/QAD.0000000000000839

Figure 2. ChIP-RT-qPCR analysis of histone modifications for the top six genes SAHA dose-responsive genes.

Figure 2

Histone modifications and Pol II occupancy were examined in the promoters of the (a) 3 most upregulated (CTNNAL1, H1F0, IRGM) and (b) 3 most downregulated (DPEP2, PHF15, SELL) SAHA dose-responsive genes when comparing a SAHA treatment of 10μM to vehicle. Fold enrichment of immunoprecipitated chromatin upon SAHA treatment is plotted on the log2 scale with error bars representing the standard error of the mean. Significant enrichment was assessed in a paired t-test and reported at the 0.1 (*) and 0.05 (**) cut-offs. Abbreviations are as follows: H3K9a, acetylated H3 Lysine 9; H4a, acetylated total Histone H4; H3K4m, methylated H3K4; H3K27m, methylated H3K27; Pol II, RNA polymerase II.