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. 2016 Jun 9;2016:9276402. doi: 10.1155/2016/9276402

Figure 2.

Figure 2

SDS-PAGE and Western blot with anti-Tg of samples from different stages of exosome isolation by ultracentrifugation: 17,000 ×g pellet and supernatant, 200,000 ×g supernatant, and 200,000 ×g (exosome pellet) from 6H medium collected from a confluent culture of FRTL-5 cells (lanes 1–4) and fresh 5H medium with 1 mg/mL bovine Tg added (lanes 5–8). Western blots were treated with a monoclonal anti-human-Tg antibody and developed using SuperSignal West Pico. “Exos” = exosomes (i.e., 200,000 ×g pellet).