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. 2016 Jun 9;2016:9848263. doi: 10.1155/2016/9848263

Figure 5.

Figure 5

Local and systemic levels of IL-1β and IL-6 were significantly attenuated in Casp11 −/− mice following infection with Toxoplasma gondii. Whole blood, peritoneal lavage fluid (PLF), and brains were collected from wild type, Casp11 −/−, and Asc −/− mice 15 days after inoculation with Toxoplasma gondii Me49 (a–d). IL-1β and IL-6 levels were determined in (a, b) serum and (c, d) cell-free PLF supernatant by ELISA. (e) IFN-γ levels were also evaluated in the cell-free PLF supernatant. (f, g) Brain samples were weighed and homogenized, and IL-1β and IL-6 levels were determined using the resultant cell-free supernatants. ∗ p < 0.05; ∗∗ p < 0.01. Wild type mock, n = 9; Asc −/− mock, n = 9; Casp11 −/− mock, n = 6; wild type Tg, n = 18; Asc −/− Tg, n = 18; Casp11 −/− Tg, n = 13. Data shown are representative of 3 independent studies.