Skip to main content
. 2016 Jun 9;2016:7920631. doi: 10.1155/2016/7920631

Figure 4.

Figure 4

Effects of ASPP2 on the protein expression of epithelial and mesenchymal markers of ARPE-19 cells. Western blot images of expression of epithelial markers (ZO-1, E-cadherin, and β-catenin) were significantly decreased, while mesenchymal markers (α-SMA, fibronectin, and ZEB1) significantly increased in ARPE-19 cells 48 h after ASPP2-siRNA transfection, when compared to the scrambled control-siRNA transfection (a, b, c, and d). β-actin or β-tubulin was used as a loading control. Representative results obtained in three independent experiments are shown. Column diagrams and bars represent the ratio of the scanned immunoblots of mesenchymal markers and epithelial markers to β-actin or β-tubulin. Data are shown as the mean ± SD, n = 3 experiments. ∗ P < 0.05 versus SC group; ∗∗ P < 0.01 versus the SC group. SC was set to 100%. SC, scrambled control-siRNA-transfected cells; ASPP2-si, ASPP2-siRNA-transfected cells.