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. 2016 Jun 23;6:28465. doi: 10.1038/srep28465

Figure 2. Inhibition of cell growth.

Figure 2

MDA-MB-231 cells treated with 4-amino acid pHLIP-KLAKS conjugates. Black columns represent cells treated at pH 7.4 and cells treated at low pH are shown in grey. For each condition, 3,000 cells/well (96-well plate) were seeded, allowed to adhere overnight, treated for 2 hours, washed once with media and cultured for 72 hour in complete medium at physiologic pH. Cell viability was assessed with the MTT assay. All measurements were normalized to the media control (0 μM, pH 7.4), as 100% cell viability. Results are shown as mean ± SEM (n = 9–12).