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. 2016 Jun 23;6:28535. doi: 10.1038/srep28535

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Time-lapse images of an embryo expressing ActTS-GR at gastrula, early neurula, and neurula stage (left), and its FRET ratio images (right). Vegetal view. Dashed line delineates the closing posterior neural plate (NP) and the lateral epidermis (LE). A: anterior. P: posterior. (b) Image of an embryo expressing hiActTS-GR at neurula stage (left) and its FRET ratio image (right). (c) Quantification of the FRET ratio in NP (oval with * in a) and LE (oval with ** in a) at the neurula stage. Values are average ± SD, n > 5. (d) Amplified image of NP (rectangle with * in a), and LE (rectangle with ** in a). Scale bars = 200 μm in a, b, 20 μm in d. *p < 0.05.