Figure 1. Rational design and purification of BsCel5A variants.

(A) Sequences of linkers showing the residues that were changed to modify linker flexibility. (B) Representation of chimeric proteins with linkers of variable length. (C) SDS-PAGE analysis of the purity of wt BsCel5A and its mutants. Purified proteins (2 μg) were loaded onto a 13% polyacrylamide gel and stained with Coomassie brilliant blue G-250.