Table 1. Kinetic parameters for the hydrolysis of β-glucan and CMC.
| β-glucan | CMC | |||||
|---|---|---|---|---|---|---|
| kcat, s−1 | KM, mg.ml−1 | kcat/KM | kcat, s−1 | KM, mg.ml−1 | kcat/KM | |
| wt | 341.37 | 2.55 ± 0.11 | 133.87 | 247.17 | 4.25 ± 0.27 | 58.16 |
| F1 | 307.41 | 2.53 ± 0.12 | 121.51 (0.91) | 241.09 | 4.24 ± 0.31 | 56.86 (0.98) |
| F2 | 277.33 | 2.54 ± 0.17 | 109.19 (0.82) | 234.11 | 4.19 ± 0.23 | 55.87 (0.96) |
| R1 | 370.14 | 2.73 ± 0.16 | 135.58 (1.04) | 257.35 | 5.86 ± 0.36 | 43.92 (0.76) |
| R2 | 353.11 | 2.69 ± 0.10 | 131.27 (1.03) | 254.23 | 5.13 ± 0.22 | 49.56 (0.85) |
| L9 | 307.90 | 2.71 ± 0.15 | 113.62 (0.85) | 189.14 | 5.85 ± 0.49 | 32.33 (0.56) |
| L56 | 344.56 | 2.58 ± 0.14 | 133.55 (1.00) | 247.30 | 4.53 ± 0.31 | 54.59 (0.94) |
| L104 | 280.61 | 2.47 ± 0.16 | 113.61 (0.85) | 178.86 | 3.86 ± 0.29 | 46.34 (0.80) |
The numbers in bold correspond to values that were significantly different from the wt enzyme (p < 0.05). The relative changes compared with wild-type BsCel5A are indicated in parentheses.