Table 2. Overall parameters calculated from the SAXS and DLS data.

Sample	Guinier Rg (Å)	Real Space Rg (Å)	Dmax (Å)	Molecular Massa (kDa)	Polydispersity (%)b
wt	34.0 ± 1.46	35.45 ± 0.05	119.0	54.71	11.6
F1	33.6 ± 1.64	34.51 ± 0.03	118.2	54.59	10.3
F2	33.4 ± 1.76	33.93 ± 0.05	116.8	54.46	8.8
R1	34.2 ± 1.13	35.75 ± 0.06	119.5	54.79	11.6
R2	33.6 ± 1.59	35.52 ± 0.09	118.7	54.77	10.8
L9	28.7 ± 1.26	28.95 ± 0.04	90.2	52.89	3.3
L56	33.6 ± 1.42	34.86 ± 0.03	117.1	57.13	13.2
L104	37.7 ± 1.35	39.42 ± 0.05	129.6	60.05	10.4

^aCalculated from the protein sequence.

^bDynamic light scattering experiments were performed at a protein concentration of 6 mg.ml⁻¹ with a DynaPro 819 at 18 °C in 50 mM sodium phosphate buffer, pH 7, 100 mM NaCl, and 5% glycerol. Polydispersity was determined with the DYNAMICS v.6.10.1.2.16 software.