Figure 3. Estradiol treatment enhances the PPARγ expression.

(A,B) Total RNA was isolated from male and female naïve T cells (CD4⁺CD62L^high) to determine the PPAR expression levels. Basal expression of PPARs in male and female naive T cells and PPARγ expression in 5 nM E2- or DMSO-treated male and female naïve T cells following TcR stimulation for 3 days were assessed using real-time PCR and were normalized to β-actin. *P < 0.05 by two-tailed, unpaired Student’s t-test. Values shown are means ± SEM (n = 3).