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. 2016 Jun 23;6:28532. doi: 10.1038/srep28532

Figure 1. Sox2 and VRK1 expression in normal epithelium and tumor cell lines.

Figure 1

(a) Expression of Sox2 and VRK1 in stratified epithelium from a normal human tonsil. VRK1 was detected with rabbit polyclonal VC1 antibody, and Sox2 was detected with murine mAb, by immunohistochemistry. (b) Sox2 and VRK1 in tonsil epithelium were detected by confocal immunofluorescence. (c) Expression of Sox2 and VRK1 protein was detected by immunoblot analysis. In MDA-MB-231 cells Sox 2 was determined after immunoprecipitation of endogenous Sox2 (bottom) with a monoclonal antibody and detected with a polyclonal antibody. (d) Colocalization of Sox2 and VRK1 in nuclei of NT2 cells by confocal microscopy. To the right is shown the profile of the signal in the plane. Similar co-localization of Sox2 and VRK1 was detected in human MCF7 and MDA-MB-231cell lines (Supplementary Fig. S1a). F: lymphoid follicle.