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. 2016 Jun 23;6:28532. doi: 10.1038/srep28532

Figure 6. VRK1 depletion upregulates SOX2 gene expression.

Figure 6

(a) In MDA-MB-231 cells, human endogenous Sox2 levels increase, when VRK1 levels decrease. 72 hours after siControl, si-VRK1-02 and si-VRK1-09 treatment, was performed the fixation and permeabilization of MDA-MB-231 cells. By immunofluorescence, human endogenous Sox2 was detected with a mouse monoclonal anti-Sox2 (E-4, 1:100). Human endogenous VRK1 was detected using a rabbit polyclonal anti-VRK1 antibody (1:200). DAPI (1:1000). In western blots, human endogenous VRK1 was detected with anti-VRK1 (1B5, 1:1000). Human endogenous Sox2 was detected using a goat polyclonal anti-Sox2 (Y-17, 1:500) and human β-actin was detected using a mAb anti-β-actin (1:5000). (b) VRK1 depletion caused a reduction of Sox2 mRNA detected by qRT-PCR. The experiment was determined three times in triplicate determinations. siCt: siControl; siV-02: siVRK1-02; siV-09: siVRK1-09.