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. 2016 Jun 23;6:28545. doi: 10.1038/srep28545

Figure 2. Regulation of npr expression by LH signaling.

Figure 2

(a) Dose dependence of hCG treatment for 2 hours on the expression of npr in the intact FG stage follicles, indicating that the expression of npr is regulated by LH signaling in vitro. (b,c) Relative expression of mprα, mprβ and npr in intact FG stage follicles of the lhβ mutant and lhr mutant, indicating that the expression of npr is regulated by LH signaling in vivo. (d) Relative expression of npr in denuded follicular cells isolated from FG stage follicles of the wild-type fish and lhβ mutant, demonstrating that the regulation of npr expression was occurrs at the follicular cells. (e) Dose response of forskolin treatment for 2 hours on the expression of npr in the primary cultured follicular cells. (f) Treatment by hCG on the expression of npr in the presence or absence of H89 in the primary cultured follicular cells, demonstrating that the cAMP-PKA pathway is involved in the regulation of npr expression by LH signaling in vitro. Each value represents the mean value ± SEM of quadruplicate assays of 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001 vs control).