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. 2016 Jun 23;6:28283. doi: 10.1038/srep28283

Figure 5. The influence of cytokine stimulation during pre-culture condition on cholangiocytic differentiation.

Figure 5

(a) Representative images of cysts derived from cultured cells with or without cytokines during 2D pre-culture. Scale bars, 100 μm. (b) The number of cysts after in vitro gel culture. The results are presented as the mean ± SEM. Statistical analysis were performed with Student’s t-test (n  =  5) (c) Flow cytometry analyses of cultured cells without cytokines. (i) Isotype control, (ii) Expression of CD326 and CD133. CD133+CD326+ cells and CD133−CD326− cells were sorted and seeded into extracellular matrix gels. CD133+CD326+ cells but not CD133−CD326− cells could form cysts in vitro (N.D, not detected). The results are presented as the mean ± SEM. Statistical analysis were performed with Student’s t-test (n  =  5). Scale bars, 100 μm. Mock, cells were pre-cultured without cytokines.