Table 1.
MS detection parameters of tryptic peptides from histone H2A and H2B.
| Modification on lysinesa |
Peptide sequence | Precursor ion (m/z) |
Product ions (m/z) |
Collision energy (eV) |
|---|---|---|---|---|
| H2A K5ac- K9ac |
4GKaQGGKaTR11 | 458.254 | 503.294, 560.315, 688.374 |
17 |
| H2A K5ac- K9un |
4GKaQGGKpTR11 | 465.262 | 517.309, 574.331, 702.389 |
17 |
| H2A K5un- K9ac |
4GKpQGGKaTR11 | 465.264 | 503.294, 560.315, 688.374 |
17 |
| H2A K5un- K9un |
4GKpQGGKpTR11 | 472.270 | 517.309, 574.331, 702.389 |
17 |
| H2A K13ac- K15ac |
12AKaAKaTR17 | 379.730 | 446.272, 517.309 |
15 |
| H2A K13ac- K15un |
12AKaAKpTR17 | 386.737 | 460.288, 531.325 |
15 |
| H2A K13un- K15ac |
12AKpAKaTR17 | 386.739 | 446.272, 517.309 |
15 |
| H2A K13un- K15un |
12AKpAKpTR17 | 393.745 | 460.288, 531.325 |
15 |
| H2A K36ac | 36KaGNYAER42 | 440.220 | 538.262, 652.305, 709.326 |
16 |
| H2A K36un | 36KpGNYAER42 | 447.227 | 538.262, 652.305, 709.326 |
16 |
| H2A K74ac- K75ac |
72DNKaKaTR77 | 423.227 | 446.272, 616.378, 730.421 |
16 |
| H2A K74ac- K75un |
72DNKaKpTR77 | 430.235 | 460.288, 630.393, 744.436 |
16 |
| H2A K74un- K75ac |
72DNKpKaTR77 | 430.237 | 446.272, 630.393, 744.436 |
16 |
| H2A K74un- K75un |
72DNKpKpTR77 | 437.243 | 460.288, 644.409, 758.452 |
16 |
| H2B K85ac | 80LAHYNKaR86 | 472.259 | 322.187, 622.331, 759.390 |
17 |
| H2B K85un | 80LAHYNKpR86 | 479.267 | 322.187, 636.346, 773.405 |
17 |
a
Acetylation and no acetylation on lysine are indicated as ac and un, respectively.