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. 2016 Jun 22;14:49. doi: 10.1186/s12951-016-0204-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — In vitro ligand dependent FRET ratio change of FLIPK in the presence of l-lysine. Affinity mutants Y14A, R77L, F52A, D161I and S72A were developed. Mutations at position 14 tyrosine substituted by alanine (Y14A), arginine 77 to leucine (R77L), phenylalanine 52 to alanine (F52A), and aspartic acid 161 to isoleucine (D161I) and serine at 72 position by alanine (S72A). Concentration of sensor protein was 0.24 mg/ml. Values are means of three independent replications. Vertical bars indicate standard error